Stool Culture and Sensitivity is a diagnostic test used to detect and identify pathogenic microorganisms (such as bacteria, viruses, and fungi) in stool that can cause gastrointestinal infections. Common pathogens include Salmonella, Shigella, E. coli, and Campylobacter, among others. The test involves culturing the stool sample in the laboratory to grow any pathogenic microorganisms present, followed by sensitivity testing to determine which antibiotics are most effective in treating the identified pathogens. This test is essential for diagnosing infections that cause symptoms like diarrhea, abdominal pain, and fever.
Patient Preparation for Stool Culture and Sensitivity
Fasting Requirements:
- Fasting: No fasting is required for this test. Patients can eat and drink normally unless instructed otherwise by their healthcare provider.
Other Preparations:
- Avoid Medications: Avoid antibiotics, antidiarrheal medications, and laxatives before the test, as they can affect the results. Inform your healthcare provider of any medications you are taking.
- Diet: No special dietary restrictions are necessary, but avoiding certain foods or substances (e.g., barium from imaging tests) may be recommended by the healthcare provider.
Sample Collection Process by Speediagno Phlebotomist or Technician:
Speediagno ensures a sterile and professional approach to collecting stool samples, adhering to strict guidelines to maintain sample integrity and accuracy.
Preparation for Stool Collection:
- Identify the Patient: The technician confirms the patient’s identity using at least two identifiers (e.g., name and date of birth).
- Consent and Explanation: The procedure is explained to the patient, including how to collect the sample properly.
Stool Collection Process:
- Collection Kit: The patient is provided with a sterile collection kit, which typically includes a clean container or collection cup, a lid, a small scoop, and instructions.
- Sample Collection:
- The patient should collect the stool sample in a clean, dry container (e.g., a disposable bedpan or directly into the sterile collection container provided).
- It’s important to avoid contamination with urine, water, or toilet paper.
- Using the scoop provided in the kit, a portion of the stool (usually about a walnut-sized amount) is transferred into the sterile container. The sample should include any parts that appear bloody, slimy, or watery, as these may contain the pathogens being tested for.
Post-Collection Care:
- Labeling and Sealing: The container is sealed tightly and labeled with the patient’s details, including the date and time of collection.
- Storage and Transport: The sample should be delivered to the laboratory as soon as possible, usually within 1-2 hours of collection. If immediate transport is not possible, the sample may be stored in a refrigerator, depending on specific instructions provided.
Safety and Hygiene Protocols:
- Proper Disposal: Any materials used during sample collection, such as gloves or containers, should be disposed of according to biohazard safety guidelines.
- Hand Hygiene: Hands should be washed thoroughly after collecting and handling the stool sample.
Laboratory Processing:
- Culture: The stool sample is cultured on selective media to grow bacteria, fungi, or other pathogens. The process usually takes 24-48 hours, though some pathogens may require longer incubation.
- Sensitivity Testing: If pathogens are identified, they undergo sensitivity testing to determine the most effective antibiotics for treatment.
This careful, hygienic approach to stool culture and sensitivity testing ensures accurate diagnosis and helps guide effective treatment for gastrointestinal infections.
All test groups and subgroup
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MOTION FOR CULTURE
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Gram staining
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Organisms Isolated
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REPORT
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Sensitivity Pattern
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Amikacin
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Cefixime
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Cefotaxime
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Co-Trimoxazole
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Cefepime
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Ciprofloxacin
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Cefoperazone/ Sulbactam
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Chloramphenicol
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Gentamicin
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Levofloxacin
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Norfloxacin
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Ofloxacin
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Piperacillin/Tazobactam
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Note
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